Properly Setting Up Koehler Alignment in a Microscope

Home
Service / Repair
Microscopes
Refractometers
FAQ
Contact Us
kohlerfigure6
Properly Setting Up Koehler Alignment in a Microscope
May 3, 2016
Facebook
Twitter
Google+
Pinterest
You have heard of the term Koehler illumination. What is it and what does it do for the microscope?

Koehler Illumination is a process which provides optimum contrast and resolution by focusing and centering the light path and spreading the light evenly over the field of view. All microscopes do not have the ability to be setup for Koehler. They must have a field diaphragm and controls to adjust the substage condenser to complete the alignment.

Using the following setup, Koehler Illumination can greatly compliment the operation of your microscope by improving the specimen contrast.

a. Rotate to the 10X objective.
b. Open the condenser aperture diaphragm with control.
c. Raise the condenser with the condenser focus knob.
d. Close the field diaphragm to it’s smallest opening with the field diaphragm control.
e. Focus the field diaphragm image with the condenser focus knob until the edges on the octagonal images are sharp.
f. Center the diaphragm image with the condenser centering screws. This centers the light path onto the specimen plane.
g. Switch to desired objective.
h. Enlarge the field diaphragm image toward edge of the field of view with the field diaphragm control.
i. Recenter the field diaphragm image.
j. Further enlarge the field diaphragm image until it is just outside the field of view. This provides enough light to fully and evenly cover the observation area.
k. Remove the right eyepiece and look down the eyepiece tube. The circular beam of light is the image of the condenser.
l. Adjust the condenser diaphragm with the condenser diaphragm control until the beam fills 3/4 of the tube (1/4 of the area will be dark and 3/4 will be light).
m. Replace the eyepiece.
n. Adjust the light intensity with the brightness control.

Setting up Koehler Illumination on your microscope will help provide the best image in an illuminated light microscope like the Meiji MT4200H or the VanGuard 1421BRI.

Leave a Reply
Your email address will not be published. Required fields are marked *

Name *

Email *

Website

CAPTCHA Image
Refresh Image

CAPTCHA Code *

Comment

You may use these HTML tags and attributes: <a href=”” title=””> <abbr title=””> <acronym title=””> <b> <blockquote cite=””> <cite> <code> <del datetime=””> <em> <i> <q cite=””> <strike> <strong>

Search for:

Recent Posts
Properly Setting Up Koehler Alignment in a Microscope
The VEE GEE Manual Polarimeter
Large Selection of Hydrometers to Choose From!
New Product Vee Gee Refractometer Model MDX
4 Highly Recommended Microscopes for Students
Categories
Hydrometer
Microscopes
National Microscope
Objective Lenses
Phase Contrast Microscope
Polarimeter
Refractometers
Supplies Kits
Technical Info
Tags
Compound Microscope Electron Microscopes Microscope Supplies Kits Objective Lenses Optical Microscopes Phase Contrast Microscope Refractometer Prism Care Refractometers Refractometers glass Stereo Microscope Types of microscopes

Products :
Refractometers
Microscopes
Polarimeters
Hydrometers
Salt Meter
Digital PH Meters
Conductivity Meters
Repair Services
Informations :
Privacy Policy
Contact Us
PAL Special Scales
Service / Repair
FAQs
Links
Conditions of Use
Shipping and Returns
Manufacturers
Articles :
Refractometers
Accessories
Microscope
Polarimeter
All Articles

CONTACT US

National Microscope Exchange

11405 West Lake Joy Drive Northeast
Carnation, Washington 98014